Composite

Part:BBa_K2569031:Design

Designed by: Dongyang Dong   Group: iGEM18_SKLMT-China   (2018-10-04)

PdnaA -NicA2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nicotine oxidase NicA2 behind our strongest constitutive promoter leading to enhance nicotine degradationin Pseudomonadaceae
compared to original nicA2 gene.

Source

NicA2 comes from the genome of Pseudomonas Putita s16. INSDC: CP002870.1.
And the constitutive promoter comes from our promoter library.

References